Fig. 4a–d.

Comparison between MosSCI using unc-119 as co-insertion marker (left) and MosSCI-biotic (right). The use of antibiotic resistance genes as co-insertion markers facilitates almost every step of MosSCI. a, b Every Mos1 recipient strain from NemaGENETAG can be used without the need to cross into an unc-119 mutant background, making host strains easier to amplify and to inject. c When using the transposase source under the control of a heat-shock promoter, large populations of nonintegrated adults can be easily obtained for heat-shock by hands-off enrichment on selective medium. d When using an antibiotic resistance gene as co-insertion marker, screening for insertion events is done in smaller populations that are not crowded by nontransgenic worms