Fig. 3.

Inhibition of the mTORC1 signaling pathway in HeLa cells transfected with mTOR siRNA. a HeLa cells were transfected with mTOR siRNA no. 4, and experiments were performed as detailed in Fig. 1a. Immunoblot analyses were carried out to determine the amount of phospho-mTOR(S2448), phospho-S6K(T389), and phospho-4EBP1(T37/46), as well as the total amount of the corresponding proteins (n = 3). b Cap-dependent translation was determined in HeLa cells transfected with both siRNA and a bicistronic reporter plasmid expressing firefly luciferase (cap-dependent translation) and Renilla luciferase (under the control of an internal ribosome entry site), as described in “Materials and methods” (n = 3). Each value represents the mean ± SD. *p < 0.05