Fig. 4.

Inhibition of the mTORC2 signaling pathway in HeLa cells transfected with mTOR siRNA. a The expression levels of phosphorylated mTOR(S2481) and AKT(S473) as well as the total amount of the corresponding proteins were examined in cells treated as described in Fig. 3a (n = 3). b FOXO1 distribution was evaluated in HeLa cells by monitoring the GFP signal 17 h after transfecting with a GFP-FOXO1 fusion protein expression vector and corresponding siRNA (n = 2). Nuclei were stained with Hoechst 33258, and images were observed by fluorescence microscopy using excitation wavelengths of 490 nm (for GFP) and 360 nm (for Hoechst). Each value represents the mean ± SD. **p < 0.01