Fig. 3.

Effect of hCG on EG-VEGF secretion. A, B Temporal (0–48 h) and dose-dependence analysis of the effect of hCG (10–100 IU/ml) on EG-VEGF secretion in placental conditioned media collected from placental explants at 6–8 wg and 9–11 wg, respectively. C Time course (0–48 h) of the effect of forskolin (10 μM) on EG-VEGF secretion. D Effects of the PKA inhibitors Rp-cAMP (100 μM) and PKI (2 μM) on the effects of hCG (25 μM) and forskolin (10 μM) on EG-VEGF secretion. The treatments lasted for 10 h. Data represent the mean ± SEM from three independent experiments (*p < 0.05, **p < 0.001)