Fig. 2.

ManNProp treatment has an axonal outgrowth-promoting effect. a, b When compared to control slices, inhibition of ST8SiaII by ManNProp had an axonal outgrowth-promoting effect, which was visible on both the suprapyramidal (arrow) and infrapyramidal (arrowhead) MF bundles. c Measurements of the MF area (left) and of the integrated signal intensity (right) of the calbindin-stained MF axons at DIV 7 confirmed the axonal outgrowth-promoting effect in ManNProp-treated cultures (n = 12) compared to control cultures (n = 15). This effect was observed for the suprapyramidal portion (supra), as well as for the infrapyramidal portion (infra) of the MF tract. Scale bar, 100 μm. *p < 0.05, **p < 0.01