Skip to main content
. 2024 May 23;19(5):e0303999. doi: 10.1371/journal.pone.0303999

Fig 1. Serine integrase mediated DNA rearrangement.

Fig 1

(A) Although the original function of this class of recombinases in nature is the integration of bacteriophage DNA into a host genome upon recombination of attB and attP sites, synthetic manipulation of att sites can lead to different outcomes. Recombination of att sites located in the same molecule will result in (B) Excision of flanked DNA region if both sites have the same orientation or (C) Inversion when sites are present in opposite orientations. (D) Recombinase Mediated Cassette Exchange (RMCE) allows the exchange of a large sequence of DNA when a molecule harbors two copies of an attP site and a second molecule harbors two copies of the cognate attB site.