Table 2. Bacterial host and sequence of attachment sites for the six integrases used by Gomide and collaborators (2020) [16].
Integrase | Host | attB (5’– 3’) | attP (5’– 3’) |
---|---|---|---|
Int2 | Streptomyces scabiei 87.22 | ggacggcgcagaaggggagtagctcttcgccggaccgtcgacatactgctcagctcgtc | gctcatgtatgtgtctacgcgagattctcgcccgagaacttctgcaaggcactgctcttggct |
Int4 | Streptococcus equi subsp equi 4047 | ttccaaagagcgcccaacgcgacctgaaatttgaataagactgctgcttgtgtaaaggcgatgatt | caaaaattacaaagttttcaacccttgatttgaattagcggtcaaataatttgtaattcgttt |
Int5 | Streptomyces phage PhiK38-1 | gagcgccggatcagggagtggacggcctgggagcgctacacgctgtggctgcggtcggtgc | ccctaatacgcaagtcgataactctcctgggagcgttgacaacttgcgcaccctgatctg |
Int7 | Geobacillus sp Y412MC61 | agacgagaaacgttccgtccgtctgggtcagttgggcaaagttgatgaccgggtcgtccgttcctt | ggtgttataaacctgtgtgagagttaagtttacatgcctaaccttaacttttacgcaggttcagctt |
Int9 | Staphylococcus aureus str. Newman | tttatattgcgaaaaataattggcgaacgaggtaactggatacctcatccgccaattaaaatttg | gtggttgtttttgttggaagtgtgtatcaggtatctgcatagttattccgaacttccaatta |
Int13 | Bacillus cytotoxicus NVH 391–98 | cgcatacattgttgttgtttttccagatccagttggtcctgtaaatataagcaatccatgtgagt | caataacggttgtatttgtagaacttgaccagttgttttagtaacataaatacaactccgaata |
Adaptation from Yang et al (2014) [15].