Table 1. Method validation parameters for the quantification of antimicrobial residues.
Target residues | LOD (μg/L) | LOQ (μg/L) | R 2 | Intra-day precision (%RSD) | Inter-day precision (%RSD) | Recovery (%) | ||
---|---|---|---|---|---|---|---|---|
Day 1 | Day 2 | Day 3 | ||||||
Ampicillin | 0.24 | 0.79 | 0.999 | 5 | 4 | 5 | 5 | 90 |
Trimethoprim | 0.04 | 0.13 | 0.994 | 3 | 1 | 3 | 11 | 101 |
Ciprofloxacin | 0.11 | 0.36 | 0.998 | 4 | 2 | 3 | 13 | 102 |
Sulfamethoxazole | 0.34 | 1.14 | 0.997 | 6 | 9 | 2 | 20 | 93 |
Fluconazole | 0.30 | 1.01 | 0.999 | 5 | 2 | 1 | 19 | 98 |
R2 = R square; LOD = limit of detection; LOQ = Limit of quantification; RSD = relative standard deviation.