Fig 6. Fluconazole susceptibility.

Wild type, efg1Δ/Δ, P_RBT5-ERG251, and efg1Δ/Δ P_RBT5-ERG251 in three strain backgrounds were assayed for fluconazole susceptibility. Strains were grown in YPD at 30°C for 16 hours with shaking, then dilutions of 5⁻² to 5⁻⁶ OD₆₀₀~3 were spotted in RPMI (pH 7.4) agar with and without fluconazole (5 μg/ml). Plates were incubated at 37°C, images were taken between 48 and 72 hours.