Figure 103.

IL-10 staining and control stainings of human PBMC. PBMC cultured for 72 h with media alone or media containing 1μM CpG-ODN 2006. The last 5 h before staining, PBMC additionally stimulated with 25 ng/ml phorbol 12-myristate 13-acetate, PMA and 1 μg/ml iono and for the last 2h with 10 μg/ml BFA (A, C) or medium control (B). Viable B cells were gated as shown in Figure 102A. IL-10⁺ B cells were gated from total viable CD19⁺ B cells. Anti-IL-10 Ab staining is shown for B cells after stimulation with PMA, iono and BFA (A) or medium control (B). Isotype control staining is shown in (C).