Figure 123.

Identification of murine NK cells in the blood and spleen of C57B/6 mice. Whole blood (A) was stained in BD Trucount tubes and analyzed after red blood cell lysis. Lymphocytes were gated among CD45⁺ leucocytes based on their morphology and, after exclusion of CD3⁺ T cells and CD19⁺ B cells, NK cells were gated as NK1.1+NKp46+ cells. For the analysis of spleen NK cells (B), due to extraction techniques, doublets and dead cells need to be gated out. CD3⁺ T cells and CD19⁺ B cells were excluded, and NK cells were gated as NK1.1+NKp46+ splenocytes.