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. Author manuscript; available in PMC: 2024 May 23.
Published in final edited form as: Eur J Immunol. 2021 Dec 7;51(12):2708–3145. doi: 10.1002/eji.202170126

Figure 141.

Figure 141.

Phenotypic characterization of HSCs from CD34-enriched human cord blood. HSPCs were identified as CD34+ CD38− cells within the human CD45+ Lin− compartment. The lineage cocktail contained the following Abs: CD3, CD10, CD14, CD15, CD16, CD19, and CD235. HSCs were identified as CD34+ CD38− CD90+ CD45RA− cells and MPPs as CD34+ CD38− CD90− CD45RA− cells [1551]. LT-HSCs with increased expansion potential can be identified within cells expressing the top 20% of Kit [1553], and LT-HSCs containing the highest repopulating activity express high levels of CD49f [1553]. Gatings for CD38 and CD90 were set according to isotype controls. Gating strategies for Kit and CD49f are chosen according to the original publications [1552, 1553]