Skip to main content
. Author manuscript; available in PMC: 2024 May 23.
Published in final edited form as: Eur J Immunol. 2021 Dec 7;51(12):2708–3145. doi: 10.1002/eji.202170126

Figure 142.

Figure 142.

Gating strategy of mouse hematopoietic stem cells. Phenotypic characterization of mouse bone marrow derived HSCs. LSK cells were identified as kit+ Sca1+ cells within the Living/Lin− compartment. LSK cells were further characterized by CD34 and Flk2 (CD135) expression. HSCs are CD150+ CD48− within the CD34−CD135− gate. MPPs are further characterized as CD150+ CD48− (MPP1) CD150+ CD48+ (MPP2) and CD150− CD48+ (MPP3) cells within the CD34+CD135− gate. MPP4 population is determined as CD34+CD135+CD150−CD48+cells. Gating for all colors were set according to the isotype control staining (not shown). Forward and side scatter voltages can be increased to dissect bone marrow cell populations into more differentiated subpopulations, differing in size and density.