Figure 145.

Flow cytometric analysis of murine neonatal astrocytes. (A) Neonatal astrocytes were harvested and stained with the cell surface marker ACSA-2 (recombinant human anti-mouse, APC-conjugated, 1:10 dilution, Miltenyi Biotec). (B) Neonatal astrocytes were harvested, fixed in 2% PFA and permeabilized in 0.5% saponin. Cells were stained with the intracellular marker GFAP (mouse monoclonal, Alexa Fluor-488-conjugated, 1:20 dilution, BD Biosciences). The gating was based on unstained controls for each Ab as shown on the far right. 10,000 cells of the SSC-A/FSC-A gate were set as a stopping point during flow cytometry. FSC and SSC axes are linear, fluorochrome axes are log.