Figure 23.

Identification and further analysis of intraepithelial lymphocytes in the human duodenum. A.- Total human intraepithelial lymphocytes (ilELs) from the duodenum were identified within singlet (97,5%) viable cells (76,7%) as CD45⁺ (34,2%), and further divided into classical T-cells (70,6%), TCRγδ T cells (27,2%) or NK-like cells (1,9%) based on the expression of CD3 and TCRγδ. This gating strategy is representative of a patient with active celiac disease, as noted by the high proportion of TCRγδ T cells coupled with the low proportion of NK-like cells. (B) The human iIEL subpopulations were further analyzed: NKG2D expression (98,6%) was measured for TCRγδ T cells, NK-like cells were divided into CD7⁺ (31,3%) and CD7⁻ cells (64,7%), and the proportion of CD8⁺ cells (74,65%), CD4⁺ cells (4,39%), double positive cells (CD4⁺CD8⁺) (2,85%) and double negative cells (CD4⁻CD8⁻) (23,8%) was assessed for T-cells.