Figure 26.

Gating strategy used to analyze markers related to differentiation, activation status, senescence, and exhaustion within human CD4⁺ T cells from a 45 y.o. healthy donor. Gating strategy is identical to that used in [126). Gate on Promokine-840 negative cells is used to exclude dead cells. Doublets are excluded based on FSC-H and FSC-W. T cells are identified based on physical parameters, and as CD45⁺CD3⁺ cells. Among CD3⁺CD4⁺ cells, naïve T cells are identified as CCR7⁺CD45RA⁺CD28⁺CD27⁺ cells; T_SCM are CCR7⁺CD45RA⁺CD28⁺CD27⁺CD95⁺; central memory (CM) are CCR7⁺CD45RA⁻CD28⁺CD27^+/−; effector memory (EM) are CCR7⁻CD45RA⁻CD28^+/−CD27^+/−; terminal effector memory (Tem) are CCR7⁻CD45RA⁺CD28⁻CD27^+/−. Activated cells are CD38⁺HLA⁻DR⁺; Treg are CD127⁻CD25⁺; exhausted/senescent are PD1⁺CD57⁺.