Figure 39.

Phenotyping of Treg cells from the murine thymus. Gating strategy to identify Treg cells in the thymus. From all events, lymphocytes can be distinguished by their FSC/SSC properties (gate G0). After lymphocyte gating, doublets are excluded twice (gates G1 and G2), followed by exclusion of dead or autofluorescent cells (gate G3). From G3, CD4SP thymocytes (gate G4) are gated, from which two Treg cell precursors (G5 and G6) and thymic Treg cells (G7) can be identified. Thymic Treg cells (G7) and CD4SP thymocytes (G4) can be subdivided into two subsets of CD24^highCD69⁺ immature (G8 and G10) and CD24^dim/lowCD69⁻ (G9 and G11) mature cells. Numbers indicate frequencies of cells within respective gates. Figures are based on thymocyte isolations from Foxp3^EGFPCreERT2ROSA26^YFP mice.