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. Author manuscript; available in PMC: 2024 May 23.
Published in final edited form as: Eur J Immunol. 2021 Dec 7;51(12):2708–3145. doi: 10.1002/eji.202170126

Figure 43.

Figure 43.

Isolation and analysis of T cells from the murine fat and lung tissue. Gating strategy to identify Treg cells in fat (A) and lung (B) tissue. From all events, a CD4-gate to identify T cells can be drawn (gate G0). Based on G0, lymphocytes can be identified by their FSC/SSC properties (gate G1). Next, doublets are excluded (gate G2) as well as unwanted, dead, or autofluorescent cells (gate G3). From G3, CD4+TCRβ+ T cells (gate G4) are gated, from which Treg cells (gate G6) and Tconv cells (gate G5) can be identified. Finally, Klrg1+ST2+ tisTregST2 (gate G7) are gated from Treg cells (gate G6). A staining of Gata-3, where numbers indicate geometric mean fluorescence intensity, exemplifies the expression of this marker in Tconv cells (gate G5, orange, dotted line), Klrg1+ST2+ tisTregST2 cells (gate G7, red), and Klrg1ST2 Treg cells (gate G8, blue). Numbers indicate frequencies of cells within respective gates. Figures are based on lung and fat digestions from Foxp3DTR, GFP animals.