Figure 46.

Expression of phenotypic markers associated with human Tr1-cells. (A) LAG3 and CD49b surface stainings of human peripheral blood of a healthy donor, in an inflamed tonsil and in the intestinal lamina propria ex vivo. (B) Ex vivo LAG3 and CD49b surface staining with a polyclonal or a monoclonal anti-LAG3 Ab on total CD4⁺ T-cells, or on gated IL-7R⁻CCR5⁺ Tr1-like cells in human peripheral blood of a healthy donor. C CCR5 and PD1 co-expression among gated CD4⁺IL-7R^lowCD25⁻T-cells allows to enrich for Tr1-like cells in different tissues. Shown is peripheral blood of a representative healthy donor. 2010 Rivino et al.