Figure 48.

Human Tr1-like cells tracked by intracellular staining for cytotoxic molecules. (A) Ex vivo GzmK and GzmB expression in gated conventional CD4⁺T-cells and FOXP3⁺Tregs. (B) GzmK and IL- 10 co-expression in FACS-purified CD4⁺IL-7R^lowCD25⁻T-cells after 4 hours of stimulation with PMA and Ionomycin. (C) Co-expression of GzmK and Eomes in FACS-purified CD4⁺IL-7R^lowCD25⁻T-cells ex vivo D: Ex vivo IL-7R and CCR6 expression patterns in among conventional CD4⁺ T-cells gated as GzmK⁺ or GzmB⁺ (see A).