Figure 5.

Detection of human circulating endothelial cells and their precursors. Gating stategy used to identify circulating endothelial cells (CEC) and their precursors (EPC) among peripheral blood leucocytes. Debris and aggregates were eliminated using FSC-Area vs FSC-Hight, while possible clogs were removed using the parameter Time vs SSC. Then, a DUMP channel was used to remove from the analysis CD45⁺ cells and dead cells. In the remaining population, nucleated cells were identified by positivity for Syto16. Stem cells were identified according to CD34 positivity, and among this population, EPC (CD133⁺,CD31⁺) and CEC (CD133⁻,CD31⁺) were identified. The expression of CD276, also named B7-H3, and CD309, also named VEGFR-2 or KDR, was evaluated in each subpopulation. In this example, more than ten million events were initially acquired.