Figure 53.

Gating strategy to define human cells γδ T cells in the peripheral blood. The gating strategy used to define human γδ T cells involves manual gating of Lymphocytes > Single Cells > Live cells > CD3⁺ cells > TCRγδ⁺. The use of γδTCR vs αβTCR mAbs provides the consistent ability to accurately discriminate γδ T cells in even the most challenging samples, i.e., where γδ T cell numbers are very low or viability is poor. γδ T cell subsets are then defined based on expression of TCRγδ⁺ > Vδ1⁺, Vδ2⁺, Vδ1/2⁻. Vδ2⁺ T cells can be further sub-divided in cells that are Vγ9⁺ or Vγ9⁻ (rare in peripheral blood).