Figure 55.

Identifying human naïve and effector sub-groups of adaptive Vγ9⁻/Vδ2⁺ and Vδ2⁻ γδ T cells in the circulation. After application of the gating strategy described in Figure 53, the distribution of clonally diverse naïve human γδ T cells can be identified by the expression of CD27 and CD45RA (CD27^hi; marked in red) and clonally expanded effector γδ T cells (CD27^lo; marked in blue), see Davey et al. [531]. These naïve and effector subsets display very distinct phenotypes and can be further defined by the expression of CX₃CR1, Granzyme A/B or IL7Rα. The data shown here is an example of the expression of these markers in human Vδ1⁺ γδ T cells.