Figure 56.

Murine pLNγδ T cells. Representative gating Lymphocytes were defined in FSC-A vs SSC-A plot. Subsequently doublets were excluded by FSC-H vs FSC-W and SSC-H vs SSC-W gating followed by exclusion of dead (Zombie-Aqua positive) and non-hematopoietic cells (CD45 negative) cells. (A) Representative contour plot for direct gating of γδ T cells defined as CD3⁺ γδ TCR⁺. (B) Representative contour plots for exclusion of αβ T cells before gating γδ T cells.