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γδ T cells in Tcrd-H2BeGFP reporter mice. Representative gating strategy for the identification of genuine γδ T cells in pLN. Lymphocytes were defined in FSC-A vs SSC-A plot. Subsequently, doublets were excluded by FSC-H vs FSC-W and SSC-H vs SSC-W gating followed by exclusion of dead (Zombie-Aqua positive) and non-hematopoietic cells (CD45 negative) cells. Genuine γδ T cells were defined based on the H2BeGFP fluorescence in Tcrd-H2BeGFP mice and counterstaining with anti-TCRβ.
