Figure 64.

Murine thymic iNKT cell populations. (A) Magnetic-bead enriched iNKT cells from C57BL/6 mice were additionally stained with Abs against CD44, NK1.1, and CD24. The upstream gating strategy is shown in NKT Figure 63. (B) Magnetic-bead enriched iNKT cells from C57BL/6 mice were additionally stained intracellulary with Abs against PLZF, T-bet and RORγt. The upstream gating strategy is shown in NKT Figure 63. (C) Magnetic-bead enriched iNKT cells from C57BL/6 mice were additionally stained with Abs against CD122 and CD4. Numbers adjacent to gates indicate frequency of parent population. The upstream gating strategy is shown in NKT Figure 63. Boldface S0, S1, S0/1, S2, S3 adjacent to gates indicate developmental stages. Boldface p, 1, 2, and 17 adjacent to gates indicate NKTp, NKT1, NKT2, and NKT17 subsets, respectively.