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. Author manuscript; available in PMC: 2024 May 23.
Published in final edited form as: Eur J Immunol. 2021 Dec 7;51(12):2708–3145. doi: 10.1002/eji.202170126

Figure 77.

Figure 77.

Versatile analysis of a murine H2-kb/SIINFEKL-specific T cell population. Double staining with nonreversible pMHC multimerized with streptavidin-PE (“Tetramer”) and reversible pMHC multimerized with streptactin-APC (“Streptamer”) before (red) and after (blue) addition of D-biotin; dissociation of Alexa488-conjugated monomeric SIINFEKL-pMHC molecules over time after addition of D-biotin (outside red box); pregating on lymphocytes, singlets, living CD19−, CD8+ CD45.2 and CD90.2-congenic coded T cells; gate of SIINFEKL-MHC-A488 additionally pregated on streptavidin-PE+ T cells.