Figure 88.

Gating strategy of cell subsets from human PBMCs. (A) Representative flow cytometry analysis of the gating strategy applied for the identification of CD8⁺ and CD4⁺ T cells from human PBMCs. Briefly, lymphocytes were first gated by the physical parameter Forward and Side scatter area (FSC-A and SSC-A) and doublets and debris were eliminated by plotting the width against the area of FSC and SSC parameters (FSC-W and SSC-W). Dead cells were excluded using Viability Dye (VD), and gating into live cells we identified CD8⁺ and CD4⁺ T cells. B) Representative FC analysis of pre- and post-enrichment of naïve (N) or effector memory plus effector memory RA⁺ (EM+EMRA) CD8⁺ T cells, gated on (dextramer⁺)-CD8⁺ T cells (upper) or (dextramer⁻)-CD8⁺ T cells (lower). (C) Representative FC analysis of pre- and post-enrichment of Treg cells with magnetic beads.