Figure 9.

Human T-cell subsets as identified by intracellular cytokine staining. (A) Expression of IFN-γ and IL-17A with or without 5 h of PMA/Ionomycin stimulation in the presence of BFA (for the last 2.5 h). (B) Expression of IFN-γ and/or IL-4, IL-17A and/or IL-22, and of IL-10 and/or GM-CSF by total CD4⁺ memory T cells. (C) T cell populations are enriched by flow cytometry according to the gating strategy indicated in Figure 2C and then stimulated in vitro for 5 h with PMA and Iono in the presence of BFA (for the last 2.5 h). Shown is the expression of IFN-γ, IL-4, and IL-17A by Th1, Th2 and Th17 cell subsets sorted ex vivo from the blood.