Media, Buffer, Staining Reagents, and Flow Cytometer	Manufacturer Information
Biocoll Seperating Solution	Ficoll-Paque Plus Cytiva #17144003
1×PBS	DPBS Lonza #17-512F
RPMI 1640 medium	Gibco #52400-025
Human AB serum	Sigma #H4522-100ML
CD40 pure – functional grade, human	Miltenyi Biotec #130-094-133
CD28 pure – functional grade, human	Miltenyi Biotec #130-093-375
Antigens of interest
Brefeldin A	Sigma Aldrich #B7651
CD154 MicroBead Kit, human	Miltenyi Biotec #130-092-658
CD137 MicroBead Kit, human	Miltenyi Biotec #130-093-476
MS columns	Miltenyi Biotec #130-042-201
MACS Separator for MS columns	Mitenyi Biotec #130-042-109
MACS MultiStand	Miltenyi Biotec #130-042-303
Bovine serum albumin (BSA) fraction	Serva #11930.04
EDTA	Promega Corporation #V4233
PEB buffer: 0,5 % BSA and 2 mM EDTA in PBS
Antibodies for surface staining
Tandem Signal Enhancer, human	Miltenyi Biotec #130-099-888
Inside Stain Kit	Miltenyi Biotec #130-090-477
Foxp3 Staining Buffer Set	Miltenyi Biotec #130-093-142
Antibodies for intracellular staining
Viobility 405/520 Fixable Dye	Miltenyi Biotec #130-110-206
LSRFortessa™	BD Biosciences