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. Author manuscript; available in PMC: 2024 May 23.
Published in final edited form as: Eur J Immunol. 2021 Dec 7;51(12):2708–3145. doi: 10.1002/eji.202170126

Table 102.

Antibodies for human monocyte identification

Target
Molecule
Fluoro
chrome
Isotype Clone Dilution Provider Cat# Target Cell Target
Tissue
Primary ab
CD3ε BV650 Mouse IgG2a OKT3 1/20 Biolegend 317324 T cell bl, sp, lu,
CD11b BV650 Rat IgG2b M1/70 1/20 Biolegend 101239 Mo/Mac lu
CD11c BV605 mouse IgG1 3.9 1/20 Biolegend 301636 cDC2 bl, sp,
CD14 BV650 mouse IgG2a M5E2 1/20 Biolegend 301836 DCs, Mac lu
CD14 Spark Blue 550 mouse IgG21 63D3 1/20 Biolegend 367147 DCs, Mo bl, sp, lu
CD14 AF700 mouse IgG2a M5E2 1/20 Biolegend 301822 Mo/Mac sp,ln
CD14 APC/Cy7 mouse IgG1 HCD14 1/20 Biolegend 325619 Mo/Mac lu
CD16 BV650 mouse IgG1 3G8 1/40 Biolegend 302018 Mo/Mac/NK cell bl, sp, lu,
CD16 APC/Cy7 mouse IgG1 3G8 1/20 Biolegend 302017 Mo/Mac ln, sp
CD16 PerCP-eFluor 710 mouse IgG1 CB16 1/20 ebioscience 46-0168-42 Mo/Mac lu
CD19 BV650 mouse IgG1 HIB19 1/20 Biolegend 302238 B cell bl, sp, lu,
CD20 BV650 mouse IgG2b 2H7 1/20 Biolegend 302336 B cell bl, sp, lu,
CD45 V500 mouse IgG1 HI30 1/20 BD Biosciences 560777 DCs, Mo/Mac bl, sp, lu,
CD45 BUV395 mouse IgG1 HI30 1/20 BD Biosciences 563792 DCs, Mo/Mac lu
CD206 PE/CFS954 mouse IgG1 19.2 1/60 BD Biosciences 564063 Mac lu
HLA-DR PE/Fire810 mouse IgG2a L243 1/20 Biolegend 307642 DCs, Mo/Mac bl, sp, lu,
HLA-DR BV785 mouse IgG2a G46-6 1/20 BD Biosciences 564041 DCs, Mo/Mac ln, sp
CD88 APC/Fire750 mouse IgG2a S5/1 1/20 Biolegend 344315 Mo/Mac
CD88 PE/vio770 mouse IgG2a S5/1 1/20 Miltenyi 130-104-336 Mo/Mac ln, sp
CD206 APC mouse IgG1 15-2 1/20 Biolegend 321109 Mac lu
LYVE1 Biotin Goat IgG Polyclonal 1/50 R&D BAF2089 Mac lu
Streptavidin PE-CF594 1/400 BD Biosciences 562284 NA lu
a)

blood (bl), spleen (sp), lung (lu), skin (sk); Mo (monocyte), Mac (macrophage)