Table 102.
Target Molecule |
Fluoro chrome |
Isotype | Clone | Dilution | Provider | Cat# | Target Cell | Target Tissue |
---|---|---|---|---|---|---|---|---|
Primary ab | ||||||||
CD3ε | BV650 | Mouse IgG2a | OKT3 | 1/20 | Biolegend | 317324 | T cell | bl, sp, lu, |
CD11b | BV650 | Rat IgG2b | M1/70 | 1/20 | Biolegend | 101239 | Mo/Mac | lu |
CD11c | BV605 | mouse IgG1 | 3.9 | 1/20 | Biolegend | 301636 | cDC2 | bl, sp, |
CD14 | BV650 | mouse IgG2a | M5E2 | 1/20 | Biolegend | 301836 | DCs, Mac | lu |
CD14 | Spark Blue 550 | mouse IgG21 | 63D3 | 1/20 | Biolegend | 367147 | DCs, Mo | bl, sp, lu |
CD14 | AF700 | mouse IgG2a | M5E2 | 1/20 | Biolegend | 301822 | Mo/Mac | sp,ln |
CD14 | APC/Cy7 | mouse IgG1 | HCD14 | 1/20 | Biolegend | 325619 | Mo/Mac | lu |
CD16 | BV650 | mouse IgG1 | 3G8 | 1/40 | Biolegend | 302018 | Mo/Mac/NK cell | bl, sp, lu, |
CD16 | APC/Cy7 | mouse IgG1 | 3G8 | 1/20 | Biolegend | 302017 | Mo/Mac | ln, sp |
CD16 | PerCP-eFluor 710 | mouse IgG1 | CB16 | 1/20 | ebioscience | 46-0168-42 | Mo/Mac | lu |
CD19 | BV650 | mouse IgG1 | HIB19 | 1/20 | Biolegend | 302238 | B cell | bl, sp, lu, |
CD20 | BV650 | mouse IgG2b | 2H7 | 1/20 | Biolegend | 302336 | B cell | bl, sp, lu, |
CD45 | V500 | mouse IgG1 | HI30 | 1/20 | BD Biosciences | 560777 | DCs, Mo/Mac | bl, sp, lu, |
CD45 | BUV395 | mouse IgG1 | HI30 | 1/20 | BD Biosciences | 563792 | DCs, Mo/Mac | lu |
CD206 | PE/CFS954 | mouse IgG1 | 19.2 | 1/60 | BD Biosciences | 564063 | Mac | lu |
HLA-DR | PE/Fire810 | mouse IgG2a | L243 | 1/20 | Biolegend | 307642 | DCs, Mo/Mac | bl, sp, lu, |
HLA-DR | BV785 | mouse IgG2a | G46-6 | 1/20 | BD Biosciences | 564041 | DCs, Mo/Mac | ln, sp |
CD88 | APC/Fire750 | mouse IgG2a | S5/1 | 1/20 | Biolegend | 344315 | Mo/Mac | |
CD88 | PE/vio770 | mouse IgG2a | S5/1 | 1/20 | Miltenyi | 130-104-336 | Mo/Mac | ln, sp |
CD206 | APC | mouse IgG1 | 15-2 | 1/20 | Biolegend | 321109 | Mac | lu |
LYVE1 | Biotin | Goat IgG | Polyclonal | 1/50 | R&D | BAF2089 | Mac | lu |
Streptavidin | PE-CF594 | − | − | 1/400 | BD Biosciences | 562284 | NA | lu |
blood (bl), spleen (sp), lung (lu), skin (sk); Mo (monocyte), Mac (macrophage)