Table 105.
Antibodies for human dendritic cell identification
| Target Molecule |
Fluoro chrome | Isotype | Clone | Dilution | Provider | Cat# | Target Cell | Target Tissue |
|---|---|---|---|---|---|---|---|---|
| Primary ab | ||||||||
| CADM1 | Purified | chicken IgY | 3E1 | 1/400 | MBL | CM004-3 | cDC1 | ln |
| CD1c | BV421 | mouse IgG1 | L161 | 1/20 | Biolegend | 331526 | cDC1,cDC2 | ln |
| CD1c | Super Bright 436 | mouse IgG1 | L161 | 1/20 | eBioscience | 62-0015-42 | cDC1,cDC2 | bl, sp |
| CD3ε | BV650 | Mouse IgG2a | OKT3 | 1/20 | Biolegend | 317324 | T cell | bl, sp, ln |
| CD5 | BV711 | mouse IgG1 | UCHT2 | 1/20 | BD Biosciences | 563170 | early pre-DC | ln |
| CD5 | APC/R700 | mouse IgG1 | UCHT2 | 1/20 | BD Biosciences | 565121 | early pre-DC | bl, sp |
| CD14 | Spark Blue 550 | mouse IgG1 | M5E2 | 1/20 | Biolegend | 367147 | DCs, Mac | bl, sp |
| CD14 | AF700 | mouse IgG2a | RMO52 | 1/10 | Beckman Coulter | IM2707U | DCs, Mo | ln |
| CD16 | BV650 | mouse IgG1 | 3G8 | 1/40 | Biolegend | 302018 | Mo/Mac/NK cell | bl, sp, ln |
| CD19 | BV650 | mouse IgG1 | HIB19 | 1/20 | Biolegend | 302238 | B cell | bl, sp, ln |
| CD20 | BV650 | mouse IgG2b | 2H7 | 1/20 | Biolegend | 302336 | B cell | bl, sp, ln |
| CD45 | V500 | mouse IgG1 | HI30 | 1/20 | BD Biosciences | 560777 | DCs, Mo/Mac | bl, sp, ln |
| CD45RA | FITC | mouse IgG2b | HI100 | 1/20 | Biolegend | 983002 | pDC, early pre-DC | ln |
| CD123 | BUV395 | mouse IgG2a | 7G3 | 1/40 | BD Biosciences | 564195 | pDC, early pre-DC | ln |
| CD123 | PE-Dazzle 594 | mouse IgG1 | S18016C | 1/20 | Biolegend | 396605 | pDC, early pre-DC | bl, sp |
| CD141 | BV421 | mouse IgG1 | 1A4 | 1/20 | BD Biosciences | 565321 | cDC1 | bl, sp |
| CD169 (Siglec-1) | PE | mouse IgG1 | 7-239 | 1/20 | BD Biosciences | 565248 | early pre-DC | ln |
| CD169 (Siglec-1) | BUV661 | mouse IgG1 | 7-239 | 1/20 | BD Biosciences | 750363 | early pre-DC | bl, sp |
| HLA-DR | BV785 | mouse IgG2a | L243 | 1/20 | Biolegend | 307642 | DCs, Mo/Mac | ln |
| HLA-DR | PE/Fire810 | mouse IgG2a | L243 | 1/20 | Biolegend | 900000155 | DCs, Mo/Mac | bl, sp |
| anti-Chicken IgY | Alexa Fluor 647 | Donkey Fab’2 | N/A | 1/200 | Jackson Immuno-research | 703-606-155 | cDC1 | ln |
| CD88 | APC/Fire750 | mouse IgG2a | S5/1 | 1/20 | Biolegend | 344315 | Mo/Mac | bl, sp |
| CD88 | PE/vio770 | mouse IgG2a | S5/1 | 1/20 | Miltenyi | 130-104-336 | Mo/Mac | ln |
| FcεR1α | BV711 | mouse IgG2a | CRA1 | 1/20 | BD Biosciences | 747783 | cDC2 | bl, sp |
| FcεR1α | PerCP/Cyanine5.5 | mouse IgG2b | CRA1 | 1/20 | Biolegend | 334621 | cDC2 | ln |
a)
blood (bl), spleen (sp), lymph node (ln); Mo (monocyte), Mac (macrophage)