Table 124.
Selection of important markers for flow cytometry analysis of human and mouse granulocytes. All cell types were first gated on CD45 and CD11b positivity
Cell type | Human | Mouse |
---|---|---|
Neutrophil | CD15pos, CD16pos, CD66bpos | Ly6Cint, Ly6Gpos |
Eosinophil | CD15pos, CD16neg, Siglec-8pos, CCR3pos, FcεRIαpos | Ly6Clow/int, Siglec-Fpos, CCR3pos, *FcεRIαpos |
Basophil | CD15neg, CD16neg, CCR3pos, FcεRIαpos, *CD117neg, *CD203cpos | Ly6Clow, CD200R3pos, CD49bpos, *FcεRIαpos |
These markers were not used in our analysis, but are valid markers for the given cell types.