Table 139.
Antibodies used for the identification of mouse HSC, MPP1, MPP2, MPP3 and MPP4 populations
| Antibody | Clone | Conjugate | Supplier |
|---|---|---|---|
| Lineage markers | |||
| CD3 | 145-2C11 | Biotin or PerCP-Cy5.5 | BD Biosciences |
| CD4 | RM4-5 | Biotin or PerCP-Cy5.5 | BioLegend |
| CD8 | 53-6.7 | Biotin or PerCP-Cy5.5 | BioLegend |
| CD11b | M1/70 | Biotin or PerCP-Cy5.5 | BioLegend |
| CD11c | N418 | Biotin or PerCP-Cy5.5 | BioLegend |
| CD19 | 6D5 | Biotin or PerCP-Cy5.5 | BioLegend |
| CD45R/B220 | RA3-6B2 | Biotin or PerCP-Cy5.5 | BioLegend |
| Ly6G/C(Gr1) | RB6-8C5 | Biotin or PerCP-Cy5.5 | BioLegend |
| NK1.1 | PK136 | Biotin or PerCP-Cy5.5 | BioLegend |
| Ter119 | TER-119 | Biotin or PerCP-Cy5.5 | BioLegend |
| Streptavidin | PerCP-Cy5.5 | BioLegend | |
| Stem and progenitor cell markers | |||
| Kit | ACK2 | APC | BioLegend |
| Sca1 | D7 | APC-Cy7 | BioLegend |
| CD135 (Flk2) | A2F10 | PE | BioLegend |
| CD34 | RAM34 | FITC | eBioscience |
| CD150 | TC15-12F12.2 | BrilliantViolet 510™ | BioLegend |
| CD48 | HM48-1 | BrilliantViolet 421™ | eBioscience |
| Isotypes control staining | |||
| Rat IgG2a κ | FITC | BioLegend | |
| Rat IgG2a κ | PE | BioLegend | |
| Armenian Hamster, Rat IgG2a/b κ, | PerCP-Cy5.5 | BioLegend | |
| Armenian Hamster | BrilliantViolet 421™ | BioLegend | |
| Rat IgG2a λ | BrilliantViolet 510™ | BioLegend | |
| Rat IgG2b κ | APC | BioLegend | |
| Rat IgG2a κ | APC-Cy7 | BioLegend | |
| Single staining for compensation controls | |||
| CD19 | 6D5 | FITC | BioLegend |
| CD19 | 1D3 | PE | BioLegend |
| CD19 | 6D5 | PerCP-Cy5.5 | BioLegend |
| CD19 | 6D5 | BrilliantViolet 421™ | BioLegend |
| CD19 | 6D5 | BrilliantViolet 510™ | BioLegend |
| CD19 | 6D5 | APC | eBioscience |
| CD19 | 6D5 | APC-Cy7 | BioLegend |
| PI (Propidium Iodide) | Invitrogen |