Table 151.

Selection of example Abs used for Kupffer cell identification in murine liver (as shown in Figure 149)

Antigen	Fluorophore	Clone	Isotype	Final concentration
CD31	BV605	390	Rat IgG2a, κ	5μg/ml
CD11b	BV650	M1/70	Rat IgG2b, κ	1μg/ml
CD45	FITC	30F11	Rat IgG2b, κ	2μg/ml
I-A/I-E (MHCII)	PerCP-Cy5.5	M5/114.15.2	Rat IgG2b, κ	2μg/ml
CD3 (Lineage)	PE-CF594	UCHT1	Mouse IgG1, κ	2μg/ml
CD19 (Lineage)	PE-CF594	1D3	Rat IgG2a, κ	1μg/ml
Ly6G (Lineage)	PE-CF594	1A8	Rat IgG2a, κ	1μg/ml
CD49b (Lineage)	PE-CF594	DX5	Rat IgM, κ	1μg/ml
TIM4	PE-Cy7	RMT4-54	Rat IgG2a, κ	1μg/ml
F4-80	APC-Cy7	BM8	Rat IgG2a, κ	2μg/ml
CD16/CD32	purified	2.4G2	Rat IgG2b, κ	5μg/ml