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. Author manuscript; available in PMC: 2024 May 23.
Published in final edited form as: Eur J Immunol. 2021 Dec 7;51(12):2708–3145. doi: 10.1002/eji.202170126
Reagent Manufacturer
OctoMACS with Heaters Miltenyi Biotec
Adult Brain Dissociation Kit Miltenyi Biotec
Neural Tissue Dissociation Kit (P) Miltenyi Biotec
C tubes Miltenyi Biotec
HBSS with Ca2+ and Mg2+ (NTDK only) Life technologies
HBSS without Ca2+ and Mg2+ (NTDK only) Life technologies
D-PBS with 0.55 mM glucose, 0.033 mM sodium pyruvate, 0.9 mM CaCl2, 0.49 mM MgCl2 (pH 7.2, keep cold) (ABDK only)
PBS + 0.5% BSA (pH 7.2, keep cold)