Table 2.

Required and facultative data for MiFlowCyt compliant cytometric publications

Data set	MiFlowCyt mandatory data	Further information, facultative
Sample/Specimen	Type, source, source treatment, phenotype, genotype, location	circumstances of sampling
Sample Treatment	Sampling, storage, preprocessing (chemical or mechanical), staining	Storage/transport temperature and humidity
Reagents	Chemicals, analytes, Antibody clones, names/numbers, manufacturer, catalogue numbers	Concentration, purity
Controls	Quality Control Measures, FMOs, Positive/negative control	For heterogeneous biocenosis individual species, if available.
Instrument	Manufacturer, model, configuration, settings, detector voltages, optical filters	If instrument is modified details on modification and performance.
Original data	Positive, negative controls, single species controls, instrument calibration measurements. Data reposition in flow repository.	Gating strategy, if applicable
Instrument	Manufacturer, model, configuration, settings, detector voltages, optical filters	If instrument is modified details on modification and performance.
Data Analysis	List-mode data file, compensation, gating, FlowRepository data access code	Software used for further analysis