Table 6.
Optical setup of the BD Fortessa used in the flow cytometry analysis
| Laser Wavelength [nm] |
Laser Power [mW] |
Laser Type |
Spectral Range for Detector [nm] |
Dichroic Filters | Band Pass Filter [nm] |
Fluorochrome | ||
|---|---|---|---|---|---|---|---|---|
| A [nm] | B [nm] | |||||||
| 633 | 40 | DPSS | 750-810 | – | 750 | 780/60 | APC Cy7 | |
| 705-755 | 750 | 690 | 730/45 | Ax700 | ||||
| 655-685 | 690 | – | 670/14 | APC | ||||
| 561 | 50 | DPSS | 750-810 | – | 750 | 780/60 | PE Cy7 | |
| 685-735 | 750 | 685 | 710/50 | Pe Cy5.5 | ||||
| 655-685 | 685 | 635 | 670/30 | Pe Cy5 | ||||
| 600-630 | 635 | 600 | 610/20 | PE TR | ||||
| 575-590 | 600 | – | 582/15 | PE | ||||
| 488 | 50 | DPSS | 685-735 | – | 685 | 710/50 | PerCP eFluor710 | |
| 515-545 | 685 | 505 | 530/30 | FITC | ||||
| 505 | – | SSC | ||||||
| 405 | 50 | DPSS | 750-810 | – | 735 | 780/60 | QD800 | |
| 685-735 | 735 | 685 | 710/50 | QD700 | ||||
| 650-670 | 685 | 635 | 660/20 | QD655 | ||||
| 600-620 | 635 | 600 | 610/20 | QD605 | ||||
| 500-550 | 600 | 505 | 525/50 | BV500 | ||||
| 425-465 | 505 | – | 450/50 | Pacific Blue / Bv421 / Alexa405 | ||||