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Panel for liquid Ab cocktail-based staining of CD25hiCD127loFOXP3+ Tregs in whole blood
| Marker | Dilution | Fluorochrome | Clone | Company | Catalogue |
|---|---|---|---|---|---|
| CD3 | 1/100 | BV786 | UCHT1 | BD | 565491 |
| CD4 | 1/100 | AF700 | RPA-T4 | BD | 557922 |
| CD25 | 1/25 | BB515 | 2A3 | BD | 564467 |
| CD127 | 1/50 | APC | A019D5 | BioLegend | 351315 |
| *FOXP3 | 1/25 | PE | 259D | BioLegend | 320207 |
| *FOXP3 | 1/25 | PE | 236A/E7 | Invitrogen | 12-4777-42 |
These FOXP3 clones are equivalent and either can be used in this panel.
