. Author manuscript; available in PMC: 2024 May 23.

Published in final edited form as: Eur J Immunol. 2021 Dec 7;51(12):2708–3145. doi: 10.1002/eji.202170126

Table 28.

List of Abs used for staining thymocytes or CD25⁺FOXP3⁺ Tregs from thymus

Marker	Dilution^**	Fluorochrome	Clone	Company	Catalogue
Fixable viability dye	1/1000	eFluor780		eBioscience	65-0865-18
CD8	1/100	FITC	HIT8a	eBioscience	11-0089-42
CD4	1/50	V500	RPA-T4	BD	560768
CD3	1/100	BV786	UCHT1	BD	565491
CD25	1/100	BV421	BC96	BioLegend	302630
FOXP3	1/50	PE	236A/E7	eBioscience	12-4777-42
Helios	1/25	AF647	22F6	BioLegend	137218

^**

These dilutions have been optimised for our specific Ab lots. Each new mAb lot should be re-titrated.