Table 53.
Characteristics of monoclonal Abs to identify human γδ T cell subsets in peripheral blood
| Buffers/Reagents for Flow Cytometry | |||||
|---|---|---|---|---|---|
| Buffer/Reagent | Provider | City | Country | Catalogue Number | Dilution |
| Lymphoprep | Axis-Shield PoC | Oslo | Denmark | NA | NA |
| Zombie Aqua | BioLegend | San Diego, CA | USA | 423101 | 1:500 |
| TruStain FcX | Biolegend | San Diego, CA | USA | 422302 | 1:100 |
| IC Fixation Buffer | eBioscience | San Diego, CA | USA | NA | 1:4 |
| Perm Buffer | eBioscience | San Diego, CA | USA | NA | 1:10 |
| Antibodies for Flow Cytometry | |||||
| mMonoclonal Ab | Fluorochrome | Clone | Provider | Catalogue Number | Dilution |
| anti-CD3 | BUV395 | UCHT1 | BD Biosciences | 563546 | 1:150 |
| anti-CD8 | BUV496 | RPA-T8 | BD Biosciences | 564804 | 1:200 |
| anti-Vγ9 | PE-Cy5 | IMMU 360 | Beckman Coulter | A63663 | 1:400 |
| anti-CD27 | PE-Dazzle 594 | M-T271 | BioLegend | 356422 | 1:150 |
| anti-CD45RA | BV711 | HI100 | BioLegend | 304138 | 1:200 |
| anti-CX3CR1 | PE-Cy7 | 2A9-1 | BioLegend | 341612 | 1:150 |
| anti-IL-7Rα | BV605 | A019D5 | BioLegend | 351333 | 1:100 |
| anti-γδTCR | PE | REA591 | Miltenyi Biotec | 130-114-038 | 1:200 |
| anti-Vδ1 | FITC | REA173 | Miltenyi Biotec | 130-100-532 | 1:100 |
| anti-Vδ2 | APC | 123R3 | Miltenyi Biotec | 130-095-803 | 1:200 |
| anti-αβTCR | APC-Vio770 | REA652 | Miltenyi Biotec | 130-114-062 | 1:100 |