Table 67.
Listing of reagents and instruments used for Figure 77
| Instrument | Multimer | Antibodies | Buffer | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Name | CyAn ADP | Streptavidin | Streptactin | SIINFEKL- MHC (Strep-tag) |
CD8 | CD45.2 | CD90.2 | CD19 | Propidium Iodid |
FACS Buffer |
| Specification/Clone | 3Laser (405,488,638nm) | Backbone-nonreversible | Backbone-reversible | pMHC-Monomer | 53-6.7 | 104 | 53-2.1 | 6D5 | Live/Dead Marker | PBS with 0.5% BSA, 1mM EDTA, 5mM D-Biotin |
| Vendor | DakoCytomation; Beckman Coulter | Bio Legend | IBA Lifesciences | MIH* | Bio Legend | Bio Legend | Life Technologies | Bio Legend | Life Technologies | Sigma Aldrich |
| Label | FSC, SSC, 9FL channels | PE | APC | Alexa Fluor 488 | PB | PerCP-Cy5.5 | APCeF780 | PEDazzle594 | PI | |
| Isotype | n.a. | n.a. | n.a. | Rat IgG2ak | Mu IgG2ak** | Rat IgG2ak | Rat IgG2ak | n.a. | ||
*
Institute for Medical Microbiology, Immunology and Hygiene
**
No Fc-block; sample material murine blood (ACT Lysed)