Table 72.

Materials for human suppression assay of Ag-specific T cells

Reagent	Fluorochrome	Clone	Company	Catalog
Lympholyte	N/A	N/A	Cedarlane	CL5010
CD8+ T Cell Isolation Kit	N/A	N/A	Miltenyi	130-096-495
Naïve CD8+ T Cell Isolation Kit	N/A	N/A	Miltenyi	130-093-244
CD4+CD25+ Regulatory T Cell Isolation Kit	N/A	N/A	Miltenyi	130-091-301
CellTrace™ CFSE Cell Proliferation Kit	CFSE	N/A	Thermo Fisher	C34554
CellTrace™ Violet Cell Proliferation Kit	Violet	N/A	Thermo Fisher	C34571
FOXP3/Transcription Factor Staining Buffer Set	N/A	N/A	eBioscience	00-5523-00
Viability dye	eFluor780	–	eBioscience	65-0865-18
Anti-CD8	Vio-Green	BW135/80 Fab	Miltenyi	BW-1 35/80
Anti-CD8	Alexa-Fluor488	SK1	BioLegend	344716
Anti-CD8	BV-510	SK1	BioLegend	344732
Anti-CD45RA	BV-605	HI100	BioLegend	304133
Anti-CCR7	PerCP-Cy5.5	G043H7	BioLegend	353220
Anti-GZMB	Bv510	GB11	BD Biosciences	563388
Anti-NKG2D	PECF594	1D11	BD Biosciences	562498
Anti-FOXP3	PerCP-Cy5.5	PCH101	eBioscience	45-4776-42
Anti-CD14	APC-eFluor780	61D3	eBioscience	47-0149-42
Anti-CD16	APC-eFluor780	CB16	eBioscience	47-0168-42
Anti-CD56	APC-eFluor780	CMSSB	eBioscience	47-0567-42
Anti-CD19	APC-eFluor780	HIB19	eBioscience	47-0199-42
Anti-CD3	PE-Cy7	SK7	BioLegend	344816
Anti-CD3	APC	SK7	BioLegend	344812
Anti-CD40L	BV-421	24-31	BioLegend	310823
Annexin V	FITC		BioLegend	640906
PI***			BioLegend	421301
Anti-CD4	Alexa-Fluor488	OKT4	eBioscience	53-0048-42
RPMI 1640 with HEPES	N/A	N/A	Gibco	22409-015
Fetal bovine serum	N/A	N/A	Gibco	10270-106
Human AB serum	N/A	N/A	Euroclone	ECS0219D
Penicillin/streptomycin	N/A	N/A	Gibco	15070-063
l-glutamine	N/A	N/A	Gibco	A29168-01
Non-essential amino acids	N/A	N/A	Euroclone	ECB3054D
Sodium pyruvate	N/A	N/A	Euroclone	ECM0542D

^***PI: Propidium Iodide