Table 75.
Antibodies and other reagents used for staining
| Antibody | Clone | Species reactivity |
isotype | Company | Dilution |
|---|---|---|---|---|---|
| IgA-AF488 | polyclonal | human | polyclonal goat IgG | Jackson Immunoresearch, West Grove PA, USA | 1:1500 |
| IgA2-PE | REA995 | human | human IgG1 | Miltenyi biotec, Bergisch Gladbach, DE | 1:100 |
| IgG1-Dylight405* | HP6188 | human | mouse IgG2b,k | Sanquin, Amsterdam, NL | 1:1000 |
| IgG2-PE/Cy5.5* | HP6014 | human | mouse IgG1,k | Sanquin, Amsterdam, NL | 1:1000 |
| IgG3-biotin* (followed by Streptavidin-PC7 | HP6095 | human | mouse IgG1,k | Sanquin, Amsterdam, NL | 1:1000 |
| IgG4-APC | SAG4 | human | mouse IgG1,k | Cytognos, Salamanca, Spain | 1:400 |
| IgM-PerCP/Cy5.5 | MHM-88 | human | mouse IgG1,k | Biolegend, San Diego, CA, USA | 1:100 |
| IgD-PE-CF594 | IA6-2 | human | mouse IgG2a,k | BD Biosciences, Franklin Lakes, NJ, USA | 1:35 |
| CD19-APC/FIRE750 | HIB19 | human | mouse IgG1,k | Biolegend, San Diego, CA, USA | 1:100 |
| CD27-BV510# | L128 | human | mIgG1,k | BD Biosciences, Franklin Lakes, NJ, USA | 1:100 |
| CD38-BV786# | HIT2 | Human | mIgG1,k | Biolegend, San Diego, CA, USA | 1:100 |
| Zombie yellow viability dye | – | – | – | Biolegend, San Diego, CA, USA | 1:100 |
| Streptavidin-PC7 | – | – | – | Biolegend, San Diego, CA, USA | 1:2000 |
*
These Abs were labeled using lightning link labeling kits from Novus Biologicals, Centennial, CO, USA.
#
These Abs were included in the staining panel but are not applied in the gating strategy shown in figure 100.