. Author manuscript; available in PMC: 2024 May 23.

Published in final edited form as: Eur J Immunol. 2021 Dec 7;51(12):2708–3145. doi: 10.1002/eji.202170126

Table 79.

Identification of murine B lineage cells in bone marrow

Subpopulation	Marker combination	Ig gene status	References
Pre-pro (Hardy A)	B220^high / CD19^neg / IgM^neg / CD43^pos	germline configuration	[1015, 1031, 1034, 1037, 1041]
	B220^pos / CD19^neg / CD43^pos / CD93^pos / CD24^neg		[1020, 1029, 1038]
^*Pro	CD19^pos / B220^pos / CD43^pos / IgM^neg / IgD^neg		[1031]
	CD19^pos / B220^pos / CD43^pos / CD93^pos / CD24^pos		[1020, 1029, 1038]
Early pro (Hardy B)	CD19^pos / B220^pos / CD43^pos / IgM^neg / CD24^pos / BP-1^neg	^**D_H - J_H	[1015, 1034, 1037]
Late pro (Hardy C)	CD19^pos / B220^pos / CD43^pos / IgM^neg / CD24^pos / BP-1^pos	V_H - DJ_H	[1013, 1015, 1034, 1037]
^*Pre (Hardy D)	CD19^pos / B220^pos / CD43^neg / IgM^neg / IgD^neg		[1015, 1031]
	CD19^pos / CD43^neg / IgM^neg / CD93^pos / CD24^pos		[1020, 1029]
^***Early pre (pre B I)	B220^pos / CD117^pos / CD25^neg	D_H - J_H	[1034, 1037]
^***Late pre (pre B II)	B220^pos / CD25^pos	V_HD_HJ_H	[1034, 1037]
Large pre	CD19^pos / B220^low / CD43^neg / CD24^pos / CD93^neg / preBcR^pos (VpreB^pos / λ5^pos)	V_HD_HJ_H	[1020, 1021, 1029, 1037]
Small pre	CD19^pos / B220^pos / CD24^pos / IgM^neg	rearrangement of the V_L-J_L starts ^**	[1020, 1029, 1037]
Immature (Hardy E)	CD19^pos / B220^pos / CD43^neg / IgM^pos / IgD^neg	Ig rearrangement complete	[1023, 1024]
	CD19^pos / B220^pos / CD24^pos / IgM^pos / IgD^neg		[1020, 1029, 1038]
Mature (Hardy F)	CD19^pos / B220^pos / CD43^neg / IgM^pos / IgD^pos		[1031]

Refers to the Hardy nomenclature.

^**

D_H, J_H, V_H, are the respective gene segments of the Ig heavy chain.

^**

V_L and J_L are the respective gene segments of the Ig light chain.

^***

Refers to the Basel nomenclature.