Fig. 7.

The Ser179Glu mutation of syndecan-4 causes failure in cytokinesis. a Different asynchronous proliferating cell cultures were monitored by phase-contrast time-lapse photography. Selected frames are shown, in which the proliferating cells are pointed by black arrows. The cells of MCF-7 (a1) and Ser179Ala expressing lines (a2) separate from the neighboring cells during rounding-up, while the cells expressing Ser179Glu SDC4 (a3) or the DEGE (a4) construct remain connected to the ex-mother cells by permanent cytoplasmic bridges shown by white arrows. Bar 25 μm. b Expression of Ser179Glu (b1) or DEGE (b2) (green) resulted in elongated, axon-like ICBs. Ser179Glu or DEGE constructs (green) were enriched in the cytoplasm and at the midbodies. The failure of abscission resulted in long thread, which connected several cells together. White arrows show the midbodies (scale bar 20 μm). c The average length of ICBs in cells expressing wt-SDC4-GFP or Ser179Glu are represented (n = 100). The length of the ICBs of Ser179Glu (45.4 ± 18.28 μm) was extended 4.5-fold compared to that of the wt-SDC4-GFP (9.8 ± 4.23 μm)