Fig. 1.

Endothelial paracellular permeability to macromolecules, a 4-kD and b 70-kD FITC-dextran, in the presence of celiac disease (CD) and control patient (non-CD)-derived antibodies as well as commercially available anti-transglutaminase 2 (TG2) antibody, CUB7402. Both types of celiac-patient derived antibodies, CD total IgA and CD anti-TG2-specific IgG, significantly increased macromolecular permeability (a, b) when compared to relevant control antibodies, while CUB7402 had no significant effect. Experiments were performed after 24 h incubation with the antibodies and the measurements were carried out 3 h after administration of the macromolecules. Experiments were performed in duplicate and repeated at least three times. Bars represent mean values and error bars standard error of means. Only significant differences (P < .05) between the relevant antibody groups are depicted