Table 2.
Other strains and plasmids used
| Strain or plasmid | Description | References or source |
|---|---|---|
| Yersinia pseudotuberculosis IP32953 | Wild type | [21] |
| Aeromonas hydrophila ATCC7966 ΔpliI::aph | pliI gene replaced by aph gene cassette from pKD4; KmR | This study |
| Escherichia coli S17-1 λpir | pro thi hsdR−hsdM+recA RP4-2-Tc::Mu-Km::Tn7 λpir | [44] |
| Escherichia coli BL21 (DE3) | Expression host for pET series vectors, containing IPTG-inducible T7 RNA polymerase gene | Novagen, Merck Biosciences, Darmstadt, Germany |
| Pichia pastoris YJT46 | Contains tjl gene of Tapes japonica under PAOX control, methanol inducible | [17] |
| pFAJ1702 | Broad host range plasmid TcR, AmpR | [45] |
| pFAJ1702-pliI | pliI from A. hydrophila ATCC7966 with its own promotor cloned in pFAJ1702; TcR, AmpR | This study |
| pET26b(+) | Expression vector | Novagen, Merck Biosciences, Darmstadt, Germany |
| pET26b(+) (PT7-pliI) | pliI from A. hydrophila under control of PT7 promotor in pET26b(+); KmR | This study |
| pDS132 | Suicide plasmid for A. hydrophila, mobilizable from E. coli S17-1; containing sacB for counterselection with sucrose; CmR | [46] |
| pDS132 (Δ pliI::aph) | pDS132 containing aph cassette flanked by regions upstream and downstream from pliI; KmR , CmR | This study |
| pKD4 | Template vector containing aph gene flanked by FRT sites, KmR, AmpR | [47] |