Table 3.

Inactivation of A. hydrophila strains by Tapes japonica lysozyme

Strain	Inhibitory activity (IU/ml) in periplasmic extract	Viability reduction factor (N0/N24)a
		1 mg/ml lactoferrin	1 mg/ml lactoferrin and 100 μg/ml TjL
A. hydrophila wild type	5.67 ± 1.14	0.634 ± 0. 423	0.342 ± 0.167
A. hydrophila ΔpliI::aph	0	0.323 ± 0.149	44.3 ± 7.85
A. hydrophilaΔpliI::aph pFAJ1702-pliI	4.04 ± 0.145	0.171 ± 0.0473	1.14 ± 0.366

^aInactivation was expressed by the viability reduction factor, with N₀ the initial colony count and N₂₄ the colony count after 24 h of incubation at 30°C. Mean values were calculated from five replicate treatments