Table 2.
Antibodies: primary and secondary antibodies and their applications/dilutions for the different analytical methods are listed
| Antigen | Supplier | Dilution | Secondary antibody | ||
|---|---|---|---|---|---|
| IF | WB | FACS | |||
| vWF | Santa Cruz, Germany | 1:500 | 1:2,500 | 1:500 | Goat anti-rabbit |
| VEGFR-2 | Cell Signaling, USA | – | 1:1,000 | 1:500 | Goat anti-rabbit |
| PECAM-1 | Cell Signaling, USA | 1:50 | 1:500 | 1:500 | Goat anti-mouse |
| ICAM-1 | Santa Cruz, Germany | – | – | 1:100 | Goat anti-mouse |
| VE-cadherin | Santa Cruz, Germany | 1:50 | 1:1,000 | 1:500 | Donkey anti-goat |
| Claudin 5 | Santa Cruz, Germany | 1:50 | 1:500 | 1:100 | Goat anti-rabbit |
| CD133 | Santa Cruz, Germany | 1:100 | 1:500 | 1:100 | Goat anti-rabbit |
| CXCR4 | Abcam | 1:100 | 1:500 | 1:100 | Goat anti-rabbit |
| CD34 | Santa Cruz, Germany | – | 1:50 | 1:100 | Goat anti-mouse |
| β-actin | Sigma, Germany | – | 1:5,000 | – | Goat anti-mouse |
| Thy-1 | AbD Serotec, Germany | 1:200 | – | 1:200 | Goat anti-mouse |
IF Immunofluorescence, WB Western blotting, and FACS fluorescence-activated cell sorting